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Material Safety Data Sheet: Lactococcus lactis

Identification

Product Name: Lactococcus lactis
Chemical Family: Lactic acid bacteria
Bacterial Strain: Various strains of Lactococcus lactis
Recommended Use: Fermentation starter for dairy, probiotic formulations, laboratory research
Manufacturer/Supplier: Common producers include biotechnology labs and dairy suppliers
Contact Information: Company-specific emergency numbers for exposure incidents or urgent questions
SDS Preparation Date: Check stamp or footer for issuing date
Synonyms: Streptococcus lactis (outdated), Lc. lactis
Form: Freeze-dried powder, culture, or liquid suspension

Hazard Identification

Classification: Not classified as hazardous under GHS for laboratory or industrial use
Physical Hazards: Non-flammable, no known physical danger
Health Hazards: May cause mild irritation to eyes, respiratory tract or skin in immune-compromised individuals or with prolonged exposure
Environmental Hazards: Not known to cause adverse environmental effects in standard use
Label Elements: No pictogram required, precautionary statements apply such as “Avoid contact with eyes and inhalation of dust”
Signal Word: None
Hazards Not Otherwise Classified: May contribute to bioaerosol formation in poorly ventilated work areas; rare allergy risk

Composition / Information on Ingredients

Component: Lactococcus lactis
CAS Number: 634-579-9 (general reference for species)
Concentration: Pure culture (100%), else with excipients (e.g., sucrose, skim milk powder) at specified percentages in blends
Impurities: Free of pathogens and contaminants as per supplier certificate; minor carryovers from fermentation media possible
Additives: Cryoprotectants such as skim milk powder or sucrose for freeze-dried forms
Physical Description: White to off-white powder or cream-colored liquid
Solubility: Suspends in water, insoluble in non-polar solvents

First Aid Measures

Inhalation: Move affected person to fresh air; seek medical advice if coughing, shortness of breath or respiratory distress occur
Ingestion: Rinse mouth, drink water; not expected to be hazardous in typical amounts but consult physician if gastrointestinal symptoms develop
Skin Contact: Wash thoroughly with soap and water; remove contaminated clothing; apply moisturizer if redness or irritation appears
Eye Contact: Rinse cautiously with water for several minutes; remove contact lenses; seek healthcare if irritation persists
Advice for Medical Personnel: Treat symptomatically; show SDS for bacterial nature of exposure
Symptoms/Effects: Respiratory allergy in sensitized persons, conjunctival redness, mild skin response possible

Fire-Fighting Measures

Suitable Extinguishing Media: Water spray, foam, dry chemical or carbon dioxide
Unsuitable Extinguishing Media: No known incompatibility with standard extinguishing agents
Hazards from Product: Product itself is non-flammable; excipient materials such as sugars or powdered milk could combust in rare fire
Protective Equipment: Firefighters need standard PPE including respiratory protection when responding to facility fires
Decomposition Products: Combustion of packaging or sugars may release smoke, carbon oxides
Firefighting Guidance: Remove product containers from fire area if safe; avoid direct contact with dust or smoke

Accidental Release Measures

Personal Precautions: Wear gloves, lab coat, eye protection, dust mask or respirator for spills or powder handling
Environmental Precautions: Prevent runoff into water systems; contain large spill with absorbent material
Methods for Clean-Up: Sweep or vacuum gently to minimize dust; dampen with water if needed; dispose as per section on disposal
Handling Precautions: Restrict access to area until cleaning is complete; avoid aerosol generation
Decontamination: Wash surface with detergent and water after material pick-up; disinfect with standard laboratory disinfectant

Handling and Storage

Safe Handling: Use in well-ventilated area; avoid inhaling dust or aerosols; wear protective clothing for repeated tasks
Hygiene Measures: Wash hands and exposed skin after handling; avoid eating or drinking in work area
Storage Conditions: Store in cool, dry environment; refrigeration required for live cultures, freezing for long-term stability
Incompatibilities: Strong acid or base, high temperature, or desiccation may reduce viability
Storage Packaging: Sealed, clearly labeled containers to prevent contamination or moisture ingress
Special Requirements: Biohazard signage only if local policies classify live cultures as such; secure storage away from incompatible chemicals

Exposure Controls and Personal Protection

Occupational Exposure Limits: Not established; control airborne levels with engineering measures
Engineering Controls: Biological safety cabinet or local exhaust ventilation for large-scale use or airborne material
Respiratory Protection: Dust mask or approved particulate respirator to reduce inhalation risk during handling or spill
Skin Protection: Laboratory gloves resistant to moisture and biological agents
Eye Protection: Safety goggles or face shield when handling powders or large liquid volumes
Other Protective Measures: Lab coat, full-length pants, closed footwear; remove and decontaminate clothing after contact

Physical and Chemical Properties

Form: Lyophilized powder, slurry, or suspension in liquid medium
Color: White to pale tan
Odor: Faint sour or dairy odor; slightly sweet in powder
pH: Neutral to slightly acidic when suspended in water (pH 5.5–7.0)
Boiling/Melting Point: Not applicable to live cultures
Flash Point: Not applicable; excipients like lactose or sugars may have high flash points in powdered form
Solubility: Dispersible in water, insoluble in oils or alcohols
Density: Depends on excipients, typically around 0.3–0.5 g/cm³ for dry preparations
Stability: Stable in dry, cool, low-light conditions
Particle Size: Finer than 200 μm for powders

Stability and Reactivity

Chemical Stability: Maintains viability in recommended storage; loss of activity from high temperatures or humidity
Reactivity: Biologically active; not chemically reactive with typical laboratory chemicals
Hazardous Reactions: None known with standard laboratory/culture materials
Materials to Avoid: Strong oxidizers or acids degrade cell viability
Decomposition Products: Lactic acid and mild volatile organic compounds as byproducts in wet storage
Hazardous Polymerization: Not known to occur

Toxicological Information

Acute Toxicity: Non-toxic to humans in handling concentrations
Chronic Exposure: No reports on chronic toxicity; extended exposure may cause mild allergies
Routes of Exposure: Inhalation, ingestion, skin and eye contact
Potential Health Effects: Very low; minor gastrointestinal effects in rare ingestion cases by immunocompromised individuals; conjunctival irritation
Carcinogenicity: Not listed by IARC, NTP, OSHA as carcinogen
Mutagenicity: No evidence present; used extensively in food fermentation for over a century
Sensitization: Low risk; possibility exists for laboratory staff with repeated exposure
Medical Conditions Aggravated: Asthma, severe immunodeficiency could increase risk from bioaerosols

Ecological Information

Ecotoxicity: No evidence of harm to aquatic or terrestrial environments
Persistence and Degradability: Natural microorganism, rapidly inactivated by sunlight, temperature, desiccation
Bioaccumulation: Unlikely, organism does not persist outside specialized environments
Mobility in Soil: Limited migration; does not proliferate aggressively in non-host environments
Degradation Products: Lactic acid, water, minor organic byproducts in environmental breakdown
Other Adverse Effects: None documented in standard agricultural or laboratory practice

Disposal Considerations

Disposal Methods: Autoclave before solid waste disposal to inactivate biological activity; follow institutional biohazard protocols
Contaminated Packaging: Dispose of as biohazardous waste; decontaminate before recycling when possible
Sewage Disposal: Discharge of small quantities possible with copious water and standard disinfectant; follow local wastewater guidelines
Special Precautions: Prevent spread into environment from large-scale spills; avoid unrestricted outdoor release
Regulatory Considerations: Adhere to applicable state and local standards for disposal of microbial cultures

Transport Information

UN Number: Not regulated under international transport agreements (IATA, DOT, ADR)
Transport Hazard Class: Non-hazardous for ground or air shipments when packed in leak-proof, secondary containment
Packing Group: Not assigned
Environmental Hazards: None expected under normal transport
Special Precautions: Refrigeration or dry-ice recommended for viability maintenance in transit; label as “live, non-pathogenic bacteria” as courtesy

Regulatory Information

OSHA Status: Non-hazardous under OSHA Hazard Communication Standard (29 CFR 1910.1200)
TSCA Status: Listed as an approved microorganism in TSCA Inventory for select uses
SARA Title III: Not subject to reporting under Section 313
FDA Status: Recognized as GRAS (Generally Recognized as Safe) for food use in US and similarly in EU
REACH Status: Exempt as naturally occurring microorganism for standard research and industrial use in EU
Other Applicable Regulations: Observe country-specific import, handling, and disposal regulations; notify biosafety officer if required