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Material Safety Data Sheet: Keratinase

Identification

Product Name: Keratinase Enzyme
Synonyms: Proteinase K, Keratin-hydrolyzing enzyme
Recommended Use: Industrial enzymatic applications, waste treatment, animal feed, leather processing
Manufacturer: Enzyme Solutions Ltd.
Contact Information: 800-555-2040, safety@enzymesolutions.com
Emergency Phone: CHEMTREC 1-800-424-9300
Address: 2040 Biotech Avenue, Houston, TX 77054
CAS Number: 9014-01-1
Product Code: KER-1002

Hazard Identification

Classification: Respiratory sensitizer (Category 1), Eye irritant (Category 2A), Skin sensitizer (Category 1)
Label Elements: Signal Word: Warning
Hazard Statements: May cause allergy or asthma symptoms, causes serious eye irritation, may cause skin irritation
Pictograms: Exclamation mark, Health hazard
Precautionary Statements: Avoid breathing dust or spray, use in well-ventilated area, wear protective gloves, eye protection
Potential Health Effects: Inhalation causes allergic reactions, prolonged contact leads to dermatitis, accidental ingestion causes mild gastrointestinal symptoms
Other Information: Persons with existing respiratory allergies report increased symptoms during exposure

Composition / Information on Ingredients

Chemical Name: Keratinase
Concentration: 80–90% by weight
Other Ingredients: Stabilizers (5–10%), Non-reactive buffer salts (3–5%)
Impurities: Residual moisture (<5%), Trace microbial proteins (<0.5%)
Molecular Formula: Variable, protein polymer
Molecular Weight: 28,000–35,000 Daltons
Formulation Details: Powder or liquid concentrate

First Aid Measures

Inhalation: Move affected person to fresh air immediately; seek medical attention for continued respiratory discomfort
Eye Contact: Rinse cautiously with water for at least 15 minutes, remove contact lenses, continue rinsing, seek advice from a healthcare professional
Skin Contact: Wash with mild soap and water, remove contaminated clothing, seek medical advice if irritation persists
Ingestion: Rinse mouth, drink water to dilute, do not induce vomiting, consult medical care if symptoms occur
General Advice: Provide medical notes on enzyme’s sensitizing potential to healthcare professionals

Fire-Fighting Measures

Suitable Extinguishing Media: Water spray, foam, dry chemical, carbon dioxide
Unsuitable Extinguishing Media: High pressure water jets (may disperse product)
Special Hazards: Combustion produces carbon oxides, nitrogen oxides, and traces of ammonia
Protective Equipment: Firefighters wear positive pressure self-contained breathing apparatus and full protective clothing
Advice for Firefighters: Cool containers with water, avoid breathing smoke, ventilate area after extinguishing
Flammability: Not considered highly flammable, but organic powder may cause dust explosion in confined spaces

Accidental Release Measures

Personal Precautions: Avoid inhalation of dust, prevent contact with skin and eyes, wear suitable personal protective equipment
Environmental Precautions: Prevent product from entering drains, surface water, or soil
Methods for Cleanup: Spill area ventilated, collect spilled material with vacuum equipped with HEPA filter, avoid dry sweeping, dispose in closed containers
Decontamination: Wash affected surfaces with water and mild detergent
Notification Procedures: Report releases according to local regulation if required

Handling and Storage

Handling: Avoid generation of aerosols, use only in properly ventilated areas, wash hands after use, keep containers tightly closed when not in use
Storage: Store at 2–8°C in original containers, keep away from food, beverages, and incompatible chemicals, protect from light and humidity
Incompatible Materials: Strong oxidizing agents, acids, bases
Special Precautions: Restrict access to trained personnel, do not reuse container, label precautions clearly

Exposure Controls and Personal Protection

Occupational Exposure Limits: No allowable exposure limit has been established, minimize airborne concentrations as low as reasonably achievable
Engineering Controls: Properly functioning local exhaust ventilation, use closed processes where possible
Personal Protective Equipment: Safety glasses with side shields, latex or nitrile gloves, laboratory coat, NIOSH-approved half mask respirator or dust mask for handling powder
Hygiene Measures: Do not eat, drink, or smoke during use, remove contaminated clothing and wash before reuse
Environmental Exposure Controls: Use spill containment and ensure work areas have spill kits available

Physical and Chemical Properties

Appearance: White to light beige powder or clear liquid concentrate
Odor: Proteinaceous, slight
pH: Solution typically pH 7–9
Melting Point/Freezing Point: Decomposes before melting
Solubility: Freely soluble in water, insoluble in organic solvents
Boiling Point: Not applicable (protein)
Density: Powder 0.6–0.8 g/cm³; Liquid 1.1–1.3 g/cm³
Flash Point: Not flammable under standard conditions
Viscosity: Liquid is moderately viscous
Partition Coefficient (n-octanol/water): Not applicable
Odor Threshold: Not determined

Stability and Reactivity

Chemical Stability: Stable under recommended storage and handling conditions
Conditions to Avoid: High temperatures, moisture, incompatible chemicals, direct sunlight
Reactivity: No hazardous reactivity under normal use
Decomposition Products: Carbon oxides, nitrogen oxides, ammonia
Incompatible Materials: Strong acids, bases, oxidizers

Toxicological Information

Acute Toxicity: Low oral and dermal toxicity in animal studies
Skin Corrosion/Irritation: May cause localized irritation with prolonged contact
Serious Eye Damage/Irritation: Causes serious eye irritation on direct exposure
Respiratory Sensitization: Enzyme dust triggers allergic reactions and asthma in sensitized individuals
Sensitization: Positive skin reactions have been noted in enzyme industry workers
Mutagenicity/Carcinogenicity: No evidence of mutagenicity or carcinogenicity in standard laboratory tests
Reproductive Toxicity: No adverse effects reported
Target Organ Effects: Lungs (inhalation), skin (prolonged contact)

Ecological Information

Ecotoxicity: Protein-based enzyme rapidly degrades in water and soil, low toxicity to aquatic organisms under normal use
Persistence and Degradability: Readily biodegradable, broken down by microbial action
Bioaccumulative Potential: No potential for bioaccumulation due to rapid degradation
Mobility in Soil: Moves with water, does not bind strongly to soil particles
Other Adverse Effects: No known long-term adverse environmental impacts with expected industrial applications

Disposal Considerations

Waste Treatment Methods: Dispose of recovered material as non-hazardous biological waste according to local, regional, national, and international regulations
Contaminated Packaging: Rinse thoroughly before recycling or disposal, follow regulations for enzyme-contaminated containers
Precautions for Disposal: Avoid disposal where material could reach surface water, notify authorities if unusual spillage occurs
Recommended Disposal: Use chemical waste incineration or licensed landfill where appropriate

Transport Information

UN Number: Not regulated for transport
UN Proper Shipping Name: Not classified as dangerous goods
Transport Hazard Classes: None
Packing Group: None
Environmental Hazards: Not a marine pollutant
Transport in Bulk: Follow storage guidelines; avoid temperature extremes
Special Precautions: Keep containers firmly sealed, clearly label contents for transport

Regulatory Information

US OSHA Status: Not hazardous under OSHA Hazard Communication Standard
TSCA Status: Exempt as naturally occurring biological substance
SARA Title III: Not listed under Section 302, 313, or 311/312
EU Classification: Complies with REACH; not classified as hazardous according to Regulation (EC) No. 1272/2008
Labelling Requirements: Indicate respiratory sensitizer on workplace safety labels
Other Regulatory Information: Handle in compliance with local, state, national, and international legislation