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Glucosidase Material Safety Data Sheet (MSDS)

Identification

Product Name: Glucosidase
Chemical Name: Beta-Glucosidase
Synonyms: Glycoside Hydrolase, Cellobiase
CAS Number: 9001-22-3
Recommended Use: Laboratory reagent, industrial enzyme, biotechnological applications
Supplier: Multiple chemical supply companies distribute glucosidase for lab and industry applications
Emergency Contact: Refer to local poison control or emergency medical services for immediate response

Hazard Identification

Classification: Not classified as hazardous under GHS criteria for most users, but repeated or excessive exposure to dust or concentrated forms can lead to irritation
Signal Word: Caution
Hazard Statements: May cause eye, skin, or respiratory irritation; may provoke allergic sensitization in some individuals exposed to enzyme powder or dust
Pictograms: Exclamation mark for irritation
Precautionary Statements: Avoid inhalation of dust, prevent eye and skin contact, use in well-ventilated areas; personal protection such as gloves and safety glasses recommended
Other Hazards: Fine powders present a dust explosion risk if allowed to accumulate in large amounts around ignition sources

Composition / Information on Ingredients

Chemical Ingredient: Beta-Glucosidase Enzyme Protein (enzyme extract derived from microbial or plant source, commonly Aspergillus niger or almonds)
Concentration: 95% or higher active enzyme (as supplied for industrial or lab use), typically mixed as a purified powder or liquid concentrate for application
Other Ingredients: Residual production medium traces (if not fully purified), stabilizers or buffer salts in solutions
Impurities: Minor residual proteins, carbohydrates, or microbial fermentation byproducts depending on manufacturing process

First Aid Measures

Inhalation: Move affected person to fresh air immediately. Seek medical assistance if symptoms such as coughing, shortness of breath, or wheezing persist.
Skin Contact: Remove contaminated clothing and rinse exposed skin with plenty of water. Wash with soap to remove any enzyme residues.
Eye Contact: Rinse eyes thoroughly with water for at least 15 minutes. Hold eyelids open and flush continuously. Obtain medical attention if irritation remains.
Ingestion: Rinse mouth and drink water if conscious. Do not provoke vomiting except on medical advice. Seek medical support where adverse effects develop.
Note for Responders: Be aware of possible allergic response if sensitized. Provide symptomatic and supportive care as required.

Fire-Fighting Measures

Suitable Extinguishing Media: Water spray, dry chemical, carbon dioxide, foam can be used depending on the fire source
Specific Hazards: Glucosidase enzyme powder can support combustion if mixed with other combustible materials or organic dust. Toxic decomposition products such as carbon oxides could form
Protective Equipment for Firefighters: Use self-contained breathing apparatus and full protective clothing in event of a major fire involving enzyme powders
Firefighting Procedures: Eliminate all ignition sources, prevent dust cloud formation, apply extinguishing media to surrounding combustibles

Accidental Release Measures

Personal Precautions: Evacuate area if airborne dust, wear respiratory protection, gloves, and eye protection to avoid exposure
Environmental Precautions: Avoid allowing large spills to enter drains, soil, or surface waters; enzyme may exhibit some ecotoxicity to aquatic organisms at high concentration
Spill Containment: Shovel or sweep up material without generating dust. Moisten spilled powder slightly if practical. Collect in appropriate containers for disposal.
Cleanup Methods: Use vacuum with HEPA filter or damp methods to clean up. Wash surfaces to deactivate any remaining enzyme residue.
Disposal: Dispose according to local, state, or national regulations as specified under "Disposal Considerations"

Handling and Storage

Handling Practices: Work in well-ventilated spaces to minimize dust or aerosol formation. Wear gloves and eye protection when manipulating concentrated forms. Clean up all dust promptly. Avoid direct contact.
Storage Conditions: Keep container tightly closed. Store in a cool, dry place. Avoid humidity and temperatures above room temperature; long-term storage at 2-8°C helps prevent enzyme degradation.
Storage Incompatibilities: Store away from strong oxidizers, acids, and bases to prevent chemical reactions. Do not store near sources of open flame or ignition.
Special Requirements: Secure containers to prevent tipping or accidental release. Restrict unauthorized access in manufacturing or bulk storage settings.

Exposure Controls and Personal Protection

Exposure Limits: No occupational exposure limit value established; keep airborne enzyme protein dust below 0.01 mg/m³ where possible
Engineering Controls: Local exhaust ventilation or fume hood to control airborne dust. Closed-handling systems desirable for large-scale operations.
Personal Protection Equipment: Nitrile gloves, safety glasses or face shield, laboratory coat recommended for all lab or plant use; N95-type dust mask or higher for airborne powder risk
Hygiene Practices: Wash hands thoroughly after handling. Do not eat, drink, or smoke in work areas.
Monitoring: Regular checks of workplace air concentration of dust, routine health monitoring for symptoms of sensitization or allergy in personnel with repeated exposure

Physical and Chemical Properties

Appearance: Off-white to pale yellow powder, or clear to slightly turbid liquid
Odor: Slight, characteristic protein/enzyme odor
pH (as supplied): Powder: not applicable; Liquid: pH 5-7 typically
Melting/Freezing Point: Not defined (degrades before melting)
Solubility: Fully soluble in water, forms clear or slightly hazy solutions
Boiling Point: Not applicable, protein denatures well below boiling point of water
Decomposition Temperature: Above 50°C to 70°C enzyme denatures and loses activity
Flash Point: Not flammable under standard conditions
Other Properties: Can generate dust clouds if handled carelessly, stable at recommended temperatures

Stability and Reactivity

Chemical Stability: Stable at ambient temperatures in dry form. Loses activity on prolonged storage above room temperature or under damp conditions.
Reactivity: No hazardous reactions in normal use; reacts with strong oxidizers, acids, alkalis.
Hazardous Decomposition Products: Emits carbon monoxide, carbon dioxide, and nitrogen oxides in fire or extreme heat
Conditions to Avoid: Avoid excessive heating, moisture, freeze-thaw cycles for liquid products.
Incompatible Materials: Strong oxidizers, acids, and alkalis
Polymerization: Does not undergo self-polymerization or hazardous reactions in storage

Toxicological Information

Acute Toxicity: Enzyme proteins generally exhibit low toxicity by oral, dermal, or inhalation routes; LD50 not established
Chronic Effects: Sensitization and allergic effects possible after repeated exposure to powder or aerosol forms; asthmatic-type symptoms can develop in sensitive individuals
Skin and Eye Contact: May cause redness, itching, irritation; repeated contact can aggravate eczema or dermatitis
Inhalation: Dust or aerosol can irritate upper respiratory tract, throat, and lungs; may provoke allergy responses
Ingestion: Low toxicity, but not recommended; ingestion may cause mild gastrointestinal symptoms
Carcinogenicity, Mutagenicity, Reproductive Toxicity: No evidence from laboratory studies or manufacturer assessments for carcinogenic, mutagenic, or reproductive effects in humans or animals

Ecological Information

Ecotoxicity: High concentrations released into aquatic environment may inhibit activity of some aquatic organisms or upset natural microbial balance
Persistence and Degradability: Enzyme products degrade rapidly in soil and water due to microbial action; not considered persistent
Bioaccumulation: No evidence of bioaccumulation due to protein nature and ready breakdown in environment
Mobility: Highly mobile in water; dilutes rapidly and decomposes in sewage or natural waterbodies
Other Adverse Effects: None reported at typical environmental exposure levels

Disposal Considerations

Waste Treatment Methods: Sweep up unused powder or collect spills and place in containers for disposal. Small quantities can be washed away with plenty of water after deactivation.
Disposal of Contaminated Packaging: Rinse empty containers thoroughly; dispose of as non-hazardous waste according to local requirements.
Special Precautions for Disposal: Prevent powder from entering watercourses, drains, or sewers in large amounts. Incineration or landfill in accordance with applicable regulations.
Regulatory Information: Consult local, national, and regional regulations for specialized disposal guidelines for industrial enzyme products.

Transport Information

UN Number: Not regulated as dangerous goods under international or domestic transport codes
Proper Shipping Name: Enzyme preparation, non-hazardous
Transport Hazard Class: Non-hazardous material
Packing Group: Not applicable
Environmental Risks: No special environmental hazards as shipped
Special Precautions: Keep containers sealed, protect from humidity, avoid crushing or rupture during transit

Regulatory Information

GHS Classification: Not classified as hazardous under GHS in most jurisdictions
Labelling Requirements: Product should display name, enzyme class, supplier identification, hazard and precautionary statements
TSCA Status: Listed or exempt in most major chemical inventories
Other National Inventories: Registered as an enzyme under US, EU, Japanese, Australian, Canadian inventories
Workplace Regulations: Enforce airborne dust guidelines, provide appropriate personal protection for workers, maintain safety data sheets on site
Other Regulations: Subject to chemical safety, occupational health, and environmental protection laws relevant to enzyme handling and manufacturing