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Product Name: Enterococcus faecalis
Other Names: Group D Streptococcus, E. faecalis
Recommended Use: Microbiology testing, laboratory reference material
Supplier Information: Laboratory or bioproduct provider address and contact details
Emergency Telephone Number: Local poison control or supplier emergency line
CAS Number: Not assigned for E. faecalis as a biological agent
Classification: Biological hazard, Biosafety Level 2 agent
Health Hazard: May cause infection if inhaled, ingested, or comes into contact with broken skin; higher risk in immunocompromised individuals
Environmental Hazard: Opportunistic pathogen in water, soil, and food
Label Elements: Biohazard symbol, warning for infectious agent
Risk Phrases: May cause serious disease in susceptible individuals, eye and skin infections, urinary tract and wound infections
Main Component: Enterococcus faecalis (live or freeze-dried strain) — whole cell or pure culture
Impurities: Product may include culture medium residues (e.g., peptone, agar, salts)
Concentration: Viable cell count depends on supplier and format; commonly 107–109 CFU/mL or g
Other Ingredients: Stabilizers for freeze-dried products (sugars, proteins)
Inhalation: Move affected person to fresh air, monitor for signs of infection over 48 hours, seek medical attention if fever or respiratory symptoms develop
Skin Contact: Wash skin thoroughly with soap and water, disinfect area, monitor for local signs of infection, medical consultation recommended for puncture wounds or skin breaks
Eye Contact: Rinse eyes gently for a minimum of 15 minutes with water, remove contact lenses if present, medical evaluation if irritation or discharge develops
Ingestion: Rinse mouth, drink water if conscious, observe for gastrointestinal symptoms, seek medical assessment if discomfort occurs
Advice for Physicians: Treat symptomatically, consider possibility of enterococcal infection, obtain cultures if fever or systemic symptoms appear
Suitable Extinguishing Media: Water spray, dry chemical, foam, or carbon dioxide
Special Hazards: Biological material; fire may destroy biological activity but may disperse particulate material; avoid inhaling smoke
Protective Equipment for Firefighters: Full protective gear, self-contained breathing apparatus, biosafety precautions for contaminated runoff
Combustion Products: Carbon monoxide, carbon dioxide, nitrogen oxides, potentially viable bacteria until incineration temperatures reached
Personal Precautions: Wear gloves, eye protection, and lab coat, use respiratory protection if material is aerosolized
Environmental Precautions: Prevent larger spills from entering drains, sewage, or water systems
Clean-up Methods: Cover spillage with paper towels soaked in appropriate disinfectant (e.g., sodium hypochlorite, 70% ethanol), allow contact time (minimum 10 minutes), carefully collect and dispose in biohazard waste
Reporting Requirements: Notify local biosafety officer or laboratory supervisor; document and log all incidents as required under institutional biosafety policy
Handling: Wear gloves, protective eyewear, and lab coat; handle inside certified biosafety cabinet if aerosol generation is likely; avoid direct contact with body surfaces; wash hands after handling
Storage Conditions: Store cultures at 2–8°C (refrigerator); maintain freeze-dried vials at recommended temperatures; keep containers tightly sealed; segregate from food and personal items
Incompatibilities: Avoid storing with highly acidic or basic chemicals, oxidizers, or food items; dedicated microbiological storage recommended
Special Storage: Clearly label with biohazard warning; limit access to qualified personnel
Engineering Controls: Certified biological safety cabinet (Class II) for manipulations likely to generate aerosols or droplets; negative pressure room if available
Personal Protective Equipment: Disinfectable lab coat or gown, single-use disposable gloves, safety goggles or face shield
Respiratory Protection: Surgical mask or N95 for aerosol-generating procedures
Hygiene Measures: Prohibit eating, drinking, or application of cosmetics in work areas; wash hands before leaving the laboratory; regular disinfection of work surfaces
Biological Exposure Monitoring: Periodic health surveillance for laboratory-acquired infections
Appearance: Creamy white to gray colonies on agar; powder or pellet in freeze-dried form
Odor: Faint, musty odor typical of bacteria grown on culture media
pH (as supplied): Neutral to slightly alkaline (7.0–8.5)
Solubility: Dispersible in water (suspension); insoluble in solvents
Stability: Stable for years when freeze-dried and stored appropriately; viable days to weeks on agar at 2–8°C
Decomposition Products: Non-volatile, unlikely to produce toxic chemicals
Chemical Stability: Stable under recommended storage conditions
Reactivity: No significant chemical reactivity; biological agent, not chemically reactive
Conditions to Avoid: Heat above 60°C for prolonged periods reduces viability
Incompatible Materials: Strong acids, bases, oxidizing agents
Hazardous Decomposition: None known in standard use; incineration at high temperatures eliminates risk
Routes of Exposure: Inhalation, contact with skin or mucous membranes, ingestion, accidental inoculation
Acute Health Effects: Fever, localized skin or eye infections, gastroenteritis, urinary tract infection especially in susceptible individuals
Chronic Health Effects: Recurrent or persistent infections may occur in immunocompromised people
Carcinogenicity: Not classified as a carcinogen
Allergenic Potential: Rarely causes allergy, but immune response possible
Symptoms of Exposure: Redness, swelling, fever, gastrointestinal upset
Mobility in Environment: Bacteria may spread in moist environments, water, soil
Persistence and Degradability: Natural bacterium, persists days to weeks in water and on surfaces; destroyed by disinfectants and heat
Bioaccumulation: Not relevant for non-spore-forming bacterium
Aquatic Toxicity: Can cause imbalance or infection in aquatic animals
Impact on Wastewater Treatment: May survive in sewage, complicate monitoring of water quality; controlled disposal crucial
Disposal of Product: Autoclave cultures, contaminated material at 121°C for 30 minutes; chemical disinfection with sodium hypochlorite or surface-active agents suitable for biological waste
Disposal of Packaging: Treat empty containers as biohazardous waste; decontaminate or autoclave before disposal
Local Regulations: Follow all institutional biosafety protocols and municipal, state, or national regulations on disposal of infectious agents
Incineration: Safe for final disposal provided approved facilities are used
UN Number: UN 3373 (Biological substance, Category B, if transported as diagnostic specimen); UN 2814/2900 for infectious substances, as applicable
Proper Shipping Name: Biological substance, category B or "Culture of Enterococcus faecalis" if regulations require
Transport Hazard Class: Class 6.2 (infectious substances)
Packaging Requirements: Triple packaging consisting of leak-proof primary receptacle, leak-proof secondary packaging, and rigid outer packaging, clearly labeled and marked
Special Precautions: Only trained personnel may handle and ship, documentation required
OSHA Hazard Symbol: Biohazard
US Federal Regulations: CDC/NIH Biosafety in Microbiological and Biomedical Laboratories (BMBL) Level 2 guidelines
EU Directives: EU Directive 2000/54/EC on biological agents at work; CLP Regulation for classification, labeling
Labeling Requirements: Clearly display biohazard symbol, risk statement, and agent details
Restriction on Use: Research, teaching, or clinical laboratories only; not for direct human use or food applications
Other Regulations: Adhere to national and local waste handling, reporting, and transportation rules for infectious substances
