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Product Name: Bacillus subtilis
Synonyms: Hay bacillus, grass bacillus
Recommended Use: Microbial inoculant, probiotic, soil and water remediation, biological preparation for agriculture and waste treatment
Manufacturer/Supplier: Name, address, and contact information of supplier
Emergency Contact: Emergency telephone number for medical information or chemical emergencies
GHS Classification: Not classified as hazardous under GHS criteria
Physical Hazards: No significant physical hazards identified
Health Hazards: Inhalation may cause respiratory irritation in sensitive users; exposure can prompt mild irritation to eyes, skin, or respiratory tract in susceptible individuals
Environmental Hazards: No significant hazard for water or soil environments
Label Elements: Not required for non-hazardous biological products
Signal Word: None
Hazard Statements: Direct contact can cause slight discomfort or mild irritation
Precautionary Statements: Avoid breathing dust, wash with water after handling, prevent eye and skin contact for those with microbial allergies
Chemical Identity: Bacillus subtilis spore suspension or powder
Common Name: Bacillus subtilis
Concentration: Viable spores typically range from 1 x 107 – 1 x 1010 CFU/g
CAS Number: 68038-70-0
Impurities/Additives: Water, inert carrier materials (such as clay or starch, if present), may contain trace protein, fermentation residues, and residual nutrients
Eye Contact: Rinse eyes cautiously with clean water for at least 15 minutes, seek medical advice if irritation persists
Skin Contact: Wash skin thoroughly with soap and water, remove contaminated clothing
Inhalation: Move exposed individual to fresh air, provide oxygen if breathing becomes difficult, consult a physician if respiratory symptoms occur
Ingestion: Rinse mouth with water and drink water if discomfort arises, seek medical attention for persistent symptoms or discomfort
Additional Advice: Show MSDS to attending medical personnel if complications occur
Suitable Extinguishing Media: Use water spray, carbon dioxide, foam, or dry chemical
Special Hazards Arising from the Substance: Product itself does not burn; packaging or added ingredients may be combustible
Protective Equipment: Firefighters should wear self-contained breathing apparatus and protective gear to prevent contact with dust or decomposition products
Firefighting Procedures: Avoid creating dust clouds, stay upwind to minimize exposure, cool surrounding containers with water spray if exposed to heat
Personal Precautions: Wear suitable respiratory protection if dust is generated, use gloves and safety goggles for extensive spills
Environmental Precautions: Prevent high concentrations from entering surface waters, storm drains, or sensitive vegetation
Containment Methods: Scoop up spill with non-sparking tools, avoid creating dust in enclosed areas
Cleanup Procedures: Collect material in sealable containers for re-use or disposal, wash area with water avoiding runoff into drains, ventilate area thoroughly
Safe Handling Advice: Minimize dust generation, avoid breathing airborne particles, use in well-ventilated locations
Hygiene Measures: Wash hands thoroughly after use, do not eat, drink, or smoke during handling, keep work areas clean
Storage Conditions: Store in cool, dry, well-ventilated area, away from direct sunlight and moisture, tightly seal original packaging
Incompatible Materials: Do not store with strong acids, alkalis, or oxidizing agents; avoid exposure to temperature extremes
Packaging Materials: Use moisture-resistant and airtight containers to protect viability and product integrity
Engineering Controls: Provide local exhaust or general ventilation to reduce airborne concentrations
Personal Protective Equipment: Use approved dust masks when risk of inhalation is present, wear protective gloves and safety glasses for large-scale handling
Respiratory Protection: Use NIOSH-approved respirators for high-exposure settings or in enclosed spaces
Eye Protection: Safety goggles recommended for splash-prone environments
Skin Protection: Protective clothing or gloves for sensitive individuals or repeated use
Environmental Exposure Control: Limit release to the environment, avoid unnecessary disposal into soil and waterways
Form: Fine powder, granules, or liquid suspension
Color: Off-white to pale beige
Odor: Slightly earthy or musty aroma
pH: 6.0 - 8.0 (1% suspension in water)
Melting/Freezing Point: Not applicable
Solubility: Insoluble in water but dispersible; spores settle upon standing
Vapor Pressure: Not applicable
Boiling Point: Not applicable
Flash Point: Not flammable
Autoignition Temperature: Not determined
Bulk Density: 0.3–0.7 g/cm3 (for powder)
Decomposition Temperature: Spores stable under typical environmental conditions, lose viability at temperatures >55°C sustained
Chemical Stability: Stable under normal handling, storage, and use conditions
Reactivity: No hazardous reactions with most storage and use conditions
Possible Hazardous Reactions: None under recommended storage and handling
Incompatible Materials: Strong acids, bases, or oxidizing agents reduce microbial viability
Hazardous Decomposition Products: Thermal decomposition can generate oxides of carbon and nitrogen
Conditions to Avoid: Excessive moisture, elevated temperatures, prolonged UV exposure, and strong chemicals
Acute Toxicity: Oral, dermal, or inhalation exposure shows low risk, minimal acute effects under normal exposure
Skin Irritation: Mild irritation possible with prolonged or repeated contact, particularly in sensitive individuals
Eye Irritation: May cause mild, transient irritation
Respiratory Sensitization: Prolonged inhalation of dust can trigger allergic symptoms such as sneezing or coughing in predisposed individuals
Chronic Exposure: No evidence of carcinogenic, mutagenic, or reproductive toxicity; safe under recommended usage
Epidemiology: Classified as Biosafety Level 1; generally regarded as safe for humans and animals
Medical Conditions Aggravated by Exposure: Asthma, chronic respiratory disorders, known allergies to bacterial proteins
Ecotoxicity: No documented toxic effects to aquatic or terrestrial plants and animals at use rates; naturally occurs in soil ecosystems
Persistence and Degradability: Spores persist short-term but return to natural baseline quickly; convertible to harmless substances
Bioaccumulation: Not bioaccumulative, integrates into native microbial ecosystems
Mobility: Spores remain localized in moist soil, minimal risk of widespread contamination
Other Adverse Effects: Benefits soil microbial health, does not disrupt non-target species at standard application rates
Waste Treatment Methods: Dispose unused material in accordance with local, regional, and national regulations
Product Disposal: Small amounts can go to general waste; larger volumes dispose as controlled biological waste
Contaminated Packaging: Clean containers thoroughly before recycling or disposal
Special Considerations: Avoid direct entry into waterways, storm drains, or public sewers
UN Number: Not regulated
UN Proper Shipping Name: Bacillus subtilis preparation
Transport Hazard Class: Not classified as hazardous for transport
Packing Group: Not applicable
Special Precautions for User: Keep containers secure and dry during transport, protect from direct sun and contaminants
Environmental Hazards: No hazard classification for transit; avoid spills
Global Regulations: Not listed as a hazardous substance by OSHA, DOT, IATA, or IMDG
TSCA: Listed
EU Regulations: Not classified as dangerous under European directives
USA Regulations: Meets the GRAS status (Generally Recognized As Safe) by the FDA for many applications
Worker Safety: No specific OSHA exposure limits; recommended to follow general hygiene and biosafety practices for microbial materials
Other Regulatory Status: Approved for use in agriculture and food in many regions; adherence to national microbial product guidelines required
