Common Name	Tween 80
CAS Number	9005-65-6
Molecular Weight	833.907
Density	1.1±0.1 g/cm3
Boiling Point	695.8±55.0 °C at 760 mmHg
Molecular Formula	C46H52N5O8P
Melting Point	-21ºC
MSDS	Chinese USA
Flash Point	207.1±25.0 °C

Density	1.1±0.1 g/cm3
Boiling Point	695.8±55.0 °C at 760 mmHg
Melting Point	-21ºC
Molecular Formula	C46H52N5O8P
Molecular Weight	833.907
Flash Point	207.1±25.0 °C
Exact Mass	833.355347
PSA	133.14000
LogP	9.39
Vapour Pressure	0.0±5.0 mmHg at 25°C
Index of Refraction	1.502
Storage condition	Store at RT.
Water Solubility	5-10 g/100 mL at 23 ºC

CHEMICAL IDENTIFICATION
RTECS NUMBER :
WG2932500
CHEMICAL NAME :
Sorbitan, monooleate polyoxyethylene deriv.
CAS REGISTRY NUMBER :
9005-65-6
LAST UPDATED :
199710
HEALTH HAZARD DATA
ACUTE TOXICITY DATA
TYPE OF TEST :
Standard Draize test
ROUTE OF EXPOSURE :
Administration into the eye
SPECIES OBSERVED :
Rodent - rabbit
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
34500 uL/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intraperitoneal
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
6804 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
1790 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
25 gm/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intraperitoneal
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
7600 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
4500 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Mammal - dog
DOSE/DURATION :
500 mg/kg
TOXIC EFFECTS :
Behavioral - altered sleep time (including change in righting reflex) Cardiac - other changes
TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Mammal - cat
DOSE/DURATION :
500 mg/kg
TOXIC EFFECTS :
Behavioral - altered sleep time (including change in righting reflex) Cardiac - other changes
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Mammal - species unspecified
DOSE/DURATION :
25 gm/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Mammal - species unspecified
DOSE/DURATION :
5800 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
42 gm/kg/2W-C
TOXIC EFFECTS :
Nutritional and Gross Metabolic - weight loss or decreased weight gain
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
2163 gm/kg/2Y-C
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria Endocrine - adrenal cortex tumors
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Subcutaneous
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
10 gm/kg/27W-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria Tumorigenic - tumors at site of application
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Oral
DOSE :
635 gm/kg
SEX/DURATION :
multigenerations
TOXIC EFFECTS :
Reproductive - Effects on Newborn - viability index (e.g., # alive at day 4 per # born alive) Reproductive - Effects on Newborn - weaning or lactation index (e.g., # alive at weaning per # alive at day 4)
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Oral
DOSE :
1270 gm/kg
SEX/DURATION :
female 84 day(s) pre-mating - 21 day(s) post-birth
TOXIC EFFECTS :
Reproductive - Effects on Newborn - viability index (e.g., # alive at day 4 per # born alive)
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Intraperitoneal
DOSE :
80 uL/kg
SEX/DURATION :
lactating female 4 day(s) post-birth
TOXIC EFFECTS :
Reproductive - Maternal Effects - uterus, cervix, vagina Reproductive - Maternal Effects - menstrual cycle changes or disorders Reproductive - Effects on Newborn - physical
MUTATION DATA
TYPE OF TEST :
DNA inhibition
TEST SYSTEM :
Rodent - mouse Cells - not otherwise specified
DOSE/DURATION :
20 ppm
REFERENCE :
ENPBBC Environmental Physiology and Biochemistry. (Copenhagen, Denmark) V.2-5, 1972-75. Discontinued. Volume(issue)/page/year: 5,84,1975 *** REVIEWS *** TOXICOLOGY REVIEW ARTODN Archives of Toxicology. (Springer-Verlag, Heidelberger Pl. 3, D-1000 Berlin 33, Fed. Rep. Ger.) V.32- 1974- Volume(issue)/page/year: 32,245,1974 *** U.S. STANDARDS AND REGULATIONS *** EPA FIFRA 1988 PESTICIDE SUBJECT TO REGISTRATION OR RE-REGISTRATION FEREAC Federal Register. (U.S. Government Printing Office, Supt. of Documents, Washington, DC 20402) V.1- 1936- Volume(issue)/page/year: 54,7740,1989 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - 80605 No. of Facilities: 1388 (estimated) No. of Industries: 18 No. of Occupations: 32 No. of Employees: 24861 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - X8582 No. of Facilities: 6365 (estimated) No. of Industries: 38 No. of Occupations: 70 No. of Employees: 162120 (estimated) No. of Female Employees: 112349 (estimated)

Personal Protective Equipment	Eyeshields;Gloves
Hazard Codes	Xi
Risk Phrases	22-36/37/38-37/38-41
Safety Phrases	S24/25
RIDADR	NONH for all modes of transport
WGK Germany	1
RTECS	WG2934000
HS Code	2932999099

N/A

1.What is Tween?

Tween® 80 is a non-ionic detergent and surfactant, classified as an ester of sorbitan and oleic acid. It is a versatile compound commonly employed in various biological applications, including cell lysis, nuclei isolation, and cell fractionation. Additionally, Tween® 80 finds use in stabilizing proteins and facilitating protein membrane studies. Its effectiveness stems from its membership in the polysorbate group, characterized by the combination of sorbitol and sorbitane oleic esters with polyethylene glycol chains. These structural features contribute to Tween® 80′s emulsifying capabilities and a broad range of applications.

2.What are the uses Tween is not suitable for？

For research and development only. Not for medicinal, domestic or other purposes.

3.What are the necessary first aid measures ?

General advice Consult a doctor. Present this safety manual to the doctor on site. If inhaled, remove the patient to fresh air. If breathing stops, give artificial respiration. Consult a doctor. Skin contact Rinse with soap and plenty of water. Consult a doctor. Eye contact Rinse thoroughly with plenty of water for at least 15 minutes and consult a doctor. ingestion Do not orally feed anything to an unconscious person. Rinse your mouth with water. Consult a doctor. General advice Consult a doctor. Present this safety manual to the doctor on site. suction If inhaled, remove the patient to fresh air. If breathing stops, give artificial respiration. Consult a doctor. Skin contact Rinse with soap and plenty of water. Consult a doctor. Eye contact Rinse thoroughly with plenty of water for at least 15 minutes and consult a doctor. ingestion Do not orally feed anything to an unconscious person. Rinse your mouth with water. Consult a doctor.

4.What is Tween used for?

Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines. TWEEN® 80 has been used in drug vehicle solution for specific injections in mice and rats. It has been used for the determination of esterase activity in bacterial cells.