Toxicity
CHEMICAL IDENTIFICATION
RTECS NUMBER :
RA1225000
CHEMICAL NAME :
Nitrous acid,
sodium salt
CAS REGISTRY NUMBER :
7632-00-0
LAST UPDATED :
199712
DATA ITEMS
CITED :
133
MOLECULAR FORMULA :
N-O2.Na
MOLECULAR WEIGHT :
69.00
WISWESSER
LINE NOTATION :
NA N-O-O
HEALTH HAZARD DATA
ACUTE TOXICITY DATA
TYPE OF TEST
:
Standard Draize test
ROUTE OF EXPOSURE :
Administration into the eye
SPECIES
OBSERVED :
Rodent - rabbit
TYPE OF TEST :
TDLo - Lowest published toxic
dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Human
DOSE/DURATION :
14
mg/kg
TOXIC EFFECTS :
Behavioral - changes in motor activity (specific assay) Vascular
- regional or general arteriolar or venous dilation Gastrointestinal - nausea or
vomiting
TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE
:
Oral
SPECIES OBSERVED :
Human - man
DOSE/DURATION :
321 mg/kg
TOXIC
EFFECTS :
Behavioral - coma Lungs, Thorax, or Respiration - cyanosis Blood -
methemoglobinemia-carboxyhemoglobin
TYPE OF TEST :
TDLo - Lowest published toxic
dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Human - man
DOSE/DURATION
:
1714 ug/kg/70M
TOXIC EFFECTS :
Cardiac - pulse rate increase, without fall in BP
Vascular - BP lowering not characterized in autonomic section
TYPE OF TEST :
LDLo -
Lowest published lethal dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED
:
Human
DOSE/DURATION :
71 mg/kg
TOXIC EFFECTS :
Behavioral - coma
Gastrointestinal - nausea or vomiting Blood - methemoglobinemia-carboxyhemoglobin
TYPE OF
TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE :
Oral
SPECIES
OBSERVED :
Human - child
DOSE/DURATION :
22 mg/kg
TOXIC EFFECTS :
Vascular -
other changes
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE
:
Oral
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
180 mg/kg
TOXIC
EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF
TEST :
LC50 - Lethal concentration, 50 percent kill
ROUTE OF EXPOSURE
:
Inhalation
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
5500
ug/m3/4H
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose
value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE
:
Subcutaneous
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
96600
ug/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose
value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE
:
Intravenous
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
65
mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose
value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE
:
Oral
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
175 mg/kg
TOXIC
EFFECTS :
Vascular - BP lowering not characterized in autonomic section Vascular -
regional or general arteriolar or venous dilation
TYPE OF TEST :
LD50 - Lethal dose,
50 percent kill
ROUTE OF EXPOSURE :
Intraperitoneal
SPECIES OBSERVED :
Rodent -
mouse
DOSE/DURATION :
158 mg/kg
TOXIC EFFECTS :
Details of toxic effects not
reported other than lethal dose value
TYPE OF TEST :
LDLo - Lowest published lethal
dose
ROUTE OF EXPOSURE :
Subcutaneous
SPECIES OBSERVED :
Rodent -
mouse
DOSE/DURATION :
150 mg/kg
TOXIC EFFECTS :
Behavioral - excitement Lungs,
Thorax, or Respiration - respiratory stimulation Gastrointestinal - nausea or
vomiting
TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE
:
Oral
SPECIES OBSERVED :
Mammal - dog
DOSE/DURATION :
330 mg/kg
TOXIC
EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF
TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE
:
Subcutaneous
SPECIES OBSERVED :
Mammal - dog
DOSE/DURATION :
60
mg/kg
TOXIC EFFECTS :
Peripheral Nerve and Sensation - flaccid paralysis without
anesthesia (usually neuromuscular blockage) Behavioral - convulsions or effect on seizure
threshold Gastrointestinal - nausea or vomiting
TYPE OF TEST :
LDLo - Lowest published
lethal dose
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Mammal -
dog
DOSE/DURATION :
15 mg/kg
TOXIC EFFECTS :
Details of toxic effects not
reported other than lethal dose value
TYPE OF TEST :
LDLo - Lowest published lethal
dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Mammal - cat
DOSE/DURATION
:
1500 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than
lethal dose value
TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF
EXPOSURE :
Subcutaneous
SPECIES OBSERVED :
Mammal - cat
DOSE/DURATION :
35
mg/kg
TOXIC EFFECTS :
Lungs, Thorax, or Respiration - dyspnea Lungs, Thorax, or
Respiration - cyanosis Gastrointestinal - changes in structure or function of salivary
glands
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE
:
Oral
SPECIES OBSERVED :
Rodent - rabbit
DOSE/DURATION :
186 mg/kg
TOXIC
EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF
TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE
:
Subcutaneous
SPECIES OBSERVED :
Rodent - rabbit
DOSE/DURATION :
60
mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose
value
TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE
:
Intravenous
SPECIES OBSERVED :
Rodent - rabbit
DOSE/DURATION :
80
mg/kg
TOXIC EFFECTS :
Peripheral Nerve and Sensation - spastic paralysis with or
without sensory change Behavioral - coma Kidney, Ureter, Bladder - urine volume
increased
TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE
:
Subcutaneous
SPECIES OBSERVED :
Amphibian - frog
DOSE/DURATION :
1
gm/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose
value
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE
:
Oral
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
22500
mg/kg/90D-C
TOXIC EFFECTS :
Liver - other changes Blood - changes in serum composition
(e.g. TP, bilirubin, cholesterol) Biochemical - Enzyme inhibition, induction, or change in
blood or tissue levels - other transferases
TYPE OF TEST :
TDLo - Lowest published
toxic dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent -
rat
DOSE/DURATION :
17080 mg/kg/61D-C
TOXIC EFFECTS :
Brain and Coverings -
changes in surface EEG Behavioral - somnolence (general depressed activity) Blood -
methemoglobinemia-carboxyhemoglobin
TYPE OF TEST :
TDLo - Lowest published toxic
dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION
:
4477 mg/kg/26W-I
TOXIC EFFECTS :
Blood - methemoglobinemia-carboxyhemoglobin
Biochemical - Enzyme inhibition, induction, or change in blood or tissue levels -
dehydrogenases Biochemical - Enzyme inhibition, induction, or change in blood or tissue
levels - transaminases
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF
EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
134
gm/kg/64W-C
TOXIC EFFECTS :
Nutritional and Gross Metabolic - weight loss or decreased
weight gain
TYPE OF TEST :
TCLo - Lowest published toxic concentration
ROUTE OF
EXPOSURE :
Inhalation
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
300
ug/m3/4H/30D-I
TOXIC EFFECTS :
Biochemical - Enzyme inhibition, induction, or change
in blood or tissue levels - phosphatases Biochemical - Enzyme inhibition, induction, or
change in blood or tissue levels - catalases
TYPE OF TEST :
TCLo - Lowest published
toxic concentration
ROUTE OF EXPOSURE :
Inhalation
SPECIES OBSERVED :
Rodent -
rat
DOSE/DURATION :
125 ug/m3/22W-I
TOXIC EFFECTS :
Brain and Coverings -
recordings from specific areas of CNS Blood - other changes Biochemical - Enzyme inhibition,
induction, or change in blood or tissue levels - phosphatases
TYPE OF TEST :
TDLo -
Lowest published toxic dose
ROUTE OF EXPOSURE :
Intraperitoneal
SPECIES OBSERVED
:
Rodent - rat
DOSE/DURATION :
960 mg/kg/30D-I
TOXIC EFFECTS :
Liver - other
changes Biochemical - Enzyme inhibition, induction, or change in blood or tissue levels -
catalases Related to Chronic Data - death
TYPE OF TEST :
TDLo - Lowest published toxic
dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION
:
185 gm/kg/61W-C
TOXIC EFFECTS :
Lungs, Thorax, or Respiration - changes in lung
weight Liver - changes in liver weight Nutritional and Gross Metabolic - weight loss or
decreased weight gain
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF
EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
2190
gm/kg/2Y-C
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Gastrointestinal - tumors
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE
OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
2149
mg/kg male 2 week(s) pre-mating female 2 week(s) pre-mating - 3 week(s) post-birth
TOXIC
EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria Reproductive - Tumorigenic effects
- transplacental tumorigenesis Lungs, Thorax, or Respiration - tumors
TYPE OF TEST
:
TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED
:
Rodent - rat
DOSE/DURATION :
63 gm/kg/95W-C
TOXIC EFFECTS :
Tumorigenic -
equivocal tumorigenic agent by RTECS criteria Gastrointestinal - tumors
TYPE OF TEST
:
TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED
:
Rodent - rat
DOSE/DURATION :
91 gm/kg/2Y-C
TOXIC EFFECTS :
Tumorigenic -
equivocal tumorigenic agent by RTECS criteria Skin and Appendages - tumors Reproductive -
Tumorigenic effects - testicular tumors
TYPE OF TEST :
TD - Toxic dose (other than
lowest)
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent -
rat
DOSE/DURATION :
183 gm/kg/2Y-C
TOXIC EFFECTS :
Tumorigenic - equivocal
tumorigenic agent by RTECS criteria Skin and Appendages - tumors Reproductive - Tumorigenic
effects - testicular tumors
TYPE OF TEST :
TD - Toxic dose (other than
lowest)
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent -
rat
DOSE/DURATION :
100 gm/kg/2Y-I
TOXIC EFFECTS :
Tumorigenic - neoplastic by
RTECS criteria Liver - tumors
TYPE OF TEST :
TD - Toxic dose (other than
lowest)
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent -
rat
DOSE/DURATION :
40 gm/kg/56W-C
TOXIC EFFECTS :
Tumorigenic - neoplastic by
RTECS criteria Liver - tumors
TYPE OF TEST :
TD - Toxic dose (other than
lowest)
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent -
rat
DOSE/DURATION :
365 gm/kg/2Y-I
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by
RTECS criteria Gastrointestinal - tumors
TYPE OF TEST :
TDLo - Lowest published toxic
dose
ROUTE OF EXPOSURE :
Oral
DOSE :
660 mg/kg
SEX/DURATION :
female 1-22
day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus -
fetal death Reproductive - Effects on Newborn - growth statistics (e.g.%, reduced weight
gain)
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE
:
Oral
DOSE :
11 gm/kg
SEX/DURATION :
female 1-22 day(s) after conception
lactating female 21 day(s) post-birth
TOXIC EFFECTS :
Reproductive - Effects on
Newborn - growth statistics (e.g.%, reduced weight gain) Reproductive - Effects on Newborn -
biochemical and metabolic Reproductive - Effects on Newborn - other postnatal measures or
effects
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE
:
Oral
DOSE :
10280 mg/kg
SEX/DURATION :
female 1-22 day(s) after conception
lactating female 20 day(s) post-birth
TOXIC EFFECTS :
Reproductive - Effects on
Newborn - weaning or lactation index (e.g., # alive at weaning per # alive at day 4)
TYPE
OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Oral
DOSE
:
6080 mg/kg
SEX/DURATION :
lactating female 20 day(s) post-birth
TOXIC EFFECTS
:
Reproductive - Effects on Newborn - growth statistics (e.g.%, reduced weight gain)
Reproductive - Effects on Newborn - other postnatal measures or effects
TYPE OF TEST
:
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Intraperitoneal
DOSE
:
400 mg/kg
SEX/DURATION :
female 10-19 day(s) after conception
TOXIC EFFECTS
:
Reproductive - Specific Developmental Abnormalities - Central Nervous System
Reproductive - Specific Developmental Abnormalities - blood and lymphatic systems (including
spleen and marrow) Reproductive - Effects on Newborn - behavioral
TYPE OF TEST :
TDLo
- Lowest published toxic dose
ROUTE OF EXPOSURE :
Oral
DOSE :
280
mg/kg
SEX/DURATION :
female 1-14 day(s) after conception
TOXIC EFFECTS
:
Reproductive - Specific Developmental Abnormalities - blood and lymphatic systems
(including spleen and marrow)
TYPE OF TEST :
TDLo - Lowest published toxic
dose
ROUTE OF EXPOSURE :
Oral
DOSE :
1200 mg/kg
SEX/DURATION :
female 6-15
day(s) after conception
TOXIC EFFECTS :
Reproductive - Fertility - pre-implantation
mortality (e.g. reduction in number of implants per female; total number of implants per
corpora lutea) Reproductive - Effects on Embryo or Fetus - fetal death
TYPE OF TEST
:
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Oral
DOSE :
1680
mg/kg
SEX/DURATION :
male 14 day(s) pre-mating
TOXIC EFFECTS :
Reproductive -
Fertility - male fertility index (e.g. # males impregnating females per # males exposed to
fertile nonpregnant females)
TYPE OF TEST :
TDLo - Lowest published toxic
dose
ROUTE OF EXPOSURE :
Oral
DOSE :
840 mg/kg
SEX/DURATION :
male 14
day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Paternal Effects - spermatogenesis
(incl. genetic material, sperm morphology, motility, and count)
TYPE OF TEST :
TDLo -
Lowest published toxic dose
ROUTE OF EXPOSURE :
Intravenous
DOSE :
36
mg/kg
SEX/DURATION :
female 30-31 week(s) after conception
TOXIC EFFECTS
:
Reproductive - Effects on Embryo or Fetus - other effects to embryo
TYPE OF TEST
:
Specific locus test
TYPE OF TEST :
Cytogenetic analysis
TYPE OF TEST
:
Cytogenetic analysis
TYPE OF TEST :
Cytogenetic analysis
TYPE OF TEST
:
Cytogenetic analysis
TYPE OF TEST :
Sister chromatid exchange
TYPE OF TEST
:
Sperm Morphology
TYPE OF TEST :
Micronucleus test
TYPE OF TEST
:
Morphological transformation
TYPE OF TEST :
Mutation in mammalian somatic
cells
MUTATION DATA
TYPE OF TEST :
DNA damage
TEST SYSTEM :
Mammal - species
unspecified Lymphocyte
DOSE/DURATION :
1 mol
REFERENCE :
JCPBAN Journal de
Chimie Physique et de Physico-Chimie Biologique. (CDR-Centrale des Revues, 11, rue Gossin,
F-92543 Montrouge Cedex, France) V.1- 1903- Volume(issue)/page/year: 58,1005,1961 ***
REVIEWS *** TOXICOLOGY REVIEW JSFAAE Journal of the Science of Food and Agriculture.
(Blackwell Scientific Pub. Inc., 52 Beacon St., Boston, MA 02108) V.1- 1950-
Volume(issue)/page/year: 26,1761,1975 TOXICOLOGY REVIEW FNSCA6 Forensic Science. (Lausanne,
Switzerland) V.1-11, 1972-78. For pub lisher information, see FSINDR.
Volume(issue)/page/year: 2,67,1973 *** U.S. STANDARDS AND REGULATIONS *** EPA FIFRA 1988
PESTICIDE SUBJECT TO REGISTRATION OR RE-REGISTRATION FEREAC Federal Register. (U.S.
Government Printing Office, Supt. of Documents, Washington, DC 20402) V.1- 1936-
Volume(issue)/page/year: 54,7740,1989 *** OCCUPATIONAL EXPOSURE LIMITS *** OEL-HUNGARY:STEL
1 mg/m3;Skin JAN 1993 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH
OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS
Hazard Code - 69230 No. of Facilities: 40763 (estimated) No. of Industries: 229 No. of
Occupations: 127 No. of Employees: 389731 (estimated) NOES - National Occupational Exposure
Survey (1983) NOES Hazard Code - 69230 No. of Facilities: 47686 (estimated) No. of
Industries: 250 No. of Occupations: 169 No. of Employees: 928532 (estimated) No. of Female
Employees: 274167 (estimated)
